comparison of embryo development between intracytoplasmic and piezo-assisted sperm injection after treating mouse sperms by ca2+ ionophore
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abstract
the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or without cai-treated sperms. results: piezo-icsi demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional icsi: 42%, p<0.001) and a cleavage rate of 73% (conventional icsi: 58%, p<0.05). when the piezo-icsi procedure was performed with cai-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, p<0.05). however, the fertilization rate did not change significantly (64% vs. 56%). conclusion: the piezo-icsi accompanies with cai-treated sperms is more efficient than the conventional icsi method for fertilizing and thus obtaining more mouse embryos.
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Journal title:
iranian biomedical journalجلد ۱۱، شماره ۴، صفحات ۲۴۵-۲۵۰
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